ECWM 2002 Abstracts

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All available 2002 East Coast Worm Meeting abstracts are listed here by number. You can download the abstracts as a PDF file.

Platform Sessions: Johnson Theater

Friday PM 7:00-10:30 GENOMICS/DISEASE MODELS

Chairs, Frank Slack and Ann Sluder

Announcements (20 minutes)

1

C. elegans SKN-1 links developmental specification of the feeding/digestive system to mechanisms that orchestrate one of its most ancestral functions, resistance to oxidative stress
Jae Hyung An, T. Keith Blackwell

2

Phytochelatin biosynthesis and transport are essential for heavy-metal detoxification in C. elegans
Olena K. Vatamaniuk, Elizabeth A. Bucher, James T. Ward, Philip A. Rea

3

APH-1 and PEN-2 are Required for Notch Pathway Signaling, gamma-Secretase Cleavage of betaAPP, and Presenilin Protein Accumulation
Ross Francis, Garth McGrath, Jianhuan Zhang, David A. Ruddy, Mary Sym, Javier Apfeld, Monique Nicoll, Mark Maxwell, Bing Hai, Michael C. Ellis, Annette L. Parks, Wei Xu, Jinhe Li, Mark Gurney, Richard L. Myers, Carol S. Himes, Ronald Hiebsch, Cara Ruble, Jeffrey S. Nye, Daniel Curtis, Richard Schumacher

4

Potent and Specific Inhibition of Calcineurin by the Down Syndrome Critical Region 1 Homologue rcn-1 in C. elegans
Jin Il Lee, Jungsoo Lee, Bijaya Dhakal, Joohong Ahnn

5

Using C. elegans to model the peroxisome biogenesis disorders
Heather Thieringer, Charles Lu, Britta Moellers, Gabriele Dodt, Wolf-H. Kunau, Monica Driscoll

6

Requirement for a p38 MAP Kinase Signaling Pathway in C. elegans Immunity
Rhonda Feinbaum, Dennis H. Kim, Hideki Inoue, Miho Tanaka-Hino, Naoki Hisamoto, Kunihiro Matsumoto, Man-Wah Tan, Frederick M. Ausubel

7

Evidence for imprinted X chromosome silencing in early C. elegans embryos.
Christopher J Bean, Christine E. Schaner, William G. Kelly

8

Dissecting the C. elegans ray developmental pathway with DNA microarrays
Douglas S. Portman, Scott W. Emmons

BREAK (30 minutes)

9

Identifying genes regulated by the TGF-beta mediated dauer pathway in Caenorhabditis elegans using cDNA microarrays
Tao Liu, John Wang, Stuart Kim, Garth I. Patterson

10

MicroRNAs in C. elegans
Nelson C. Lau, Lee P. Lim, Earl G. Weinstein, Chris B. Burge, David P. Bartel

11

IDENTIFICATION AND FUNCTIONAL ANALYSIS OF microRNA GENES IN C. elegans AND D. melanogaster.
Allison L. Abbott, Victor Ambros, Daniel Hopkins, David Jewell, Ann Lavanway, Rosalind C. Lee, Lorenzo Sempere, Nicholas S. Sokol, Peter T. Williams

12

Evidence that the Heterochronic Gene lin-58 is a MicroRNA Homologous to the let-7 Small Temporal RNA
Ming Li, Matthew W. Rhoades, Nelson C. Lau, David P. Bartel, Ann E. Rougvie

13

An RNAi-based screen identifies putative mediators and targets of the let-7 small temporal RNA
Helge Grosshans, Frank J Slack

14

New components of the RNAi pathway in C. elegans
Erbay Yigit, Martin Simard, Hiroaki Tabara, Craig C. Mello

15

At least two target-dependent events are required for RNA interference in C elegans
Darryl Conte, Jr, Alla Grishok, Chun-Chieh Chen, Hiroaki Tabara, Craig C Mello

16

THE C. ELEGANS ORFEOME CLONING PROJECT : VERSION 1.0
Jérôme Reboul, Philippe Vaglio, Jean-François Rual, Philippe Lamesch, Laurent Jacotot, Monica Martinez, Christopher Armstrong, Nicolas Bertin, Alban Chesneau, Troy Moore, Jim Hartley, Mike Brasch, Hongmei Lee, Lynn Doucette-Stamm, David E Hill, Marc Vidal

Saturday AM 8:30-12:30 DEVELOPMENT

Chair, Richard Roy and Shai Shaham

17

Exclusion of germ plasm components from somatic lineages by localized protein degradation.
Cynthia L. DeRenzo, Kimberly J. Reese, Geraldine C. Seydoux

18

Post-PIE-1 Chromatin Reorganization in the Embryonic Germline of C. elegans
Christine E. Schaner, William G. Kelly

19

Sperm cues and polarization of the anterior-posterior axis in C. elegans
Rebecca Lyczak, Sarah Kirkegaard, Bruce Bowerman

20

Temporal and spatial control of initial meiotic entry in the C. elegans germ line
Anita S.-R. Pepper, Darrell J. Killian, Te-Wen Lo, E. Jane Albert Hubbard

21

Germline-Soma Distinction in C. elegans Required the Zinc-Finger Protein MEP-1 and LET-418/Mi2, a Conserved Component of the Nucleosome Remodeling and Histone Deacetylase Complex
Yingdee Unhavaithaya, Tae Ho Shin, Nicholas miliaras, Jong Soon Lee, Craig Mello

22

GLD-1 and the pal-1 3’ UTR are implicated in the post-initiation translational repression of maternal pal-1 mRNA in C. elegans
Darcy E. Mootz, Diana Ho, Craig P. Hunter

23

The C. elegans Schnurri homolog SMA-9 in postembryonic mesodermal cell fate specification
Jun Liu, Jun Liang, Judith Yanowitz, Cathy Savage-Dunn, Andrew Fire

24

Acetylation regulates the subcellular localization of POP-1, a C. elegans cell fate-determining transcription factor
Frédérique Gay, Dominica Calvo, Miao-Chia Lo, Julian Ceron, Rueyling Lin, Yang Shi

25

egl-41, which may act in sex determination, is identical to sel-10, which encodes an F-box protein and negative regulator of lin-12/Notch
Sibylle Jäger, Hillel Schwartz, Bob Horvitz, Barbara Conradt

26

Molecular connections between developmental timing and circadian timing: The C. elegans homologues of the circadian genes doubletime and timeless regulate post-embryonic developmental timing
Diya Banerjee, Alvin Kwok, Shin-Yi Lin, Frank Slack

BREAK (30 minutes)

27

A Pc-G like gene, sop-2, is required for maintaining the repressed state of Hox genes in C. elegans
Hong Zhang, Ricardo Azevedo, Robyn Lints, Christina Doyle, Yingqi Teng, Daniel Haber, Scott Emmons

28

A NEW CLASS OF SYNMUV MUTATIONS IS PREDICTED TO DISRUPT A HISTONE ACETYLTRANSFERASE COMPLEX
Craig Ceol, Bob Horvitz

29

eor-1 and eor-2 act downstream of the Ras and Wnt pathways during vulval and P12 development
Robyn M. Howard, Meera V. Sundaram

30

SQV-4 UDP-GLUCOSE DEHYDROGENASE IS TEMPORALLY AND SPATIALLY REGULATED TO CONTROL C. ELEGANS VULVAL MORPHOGENESIS
Ho-Yon Hwang, Bob Horvitz

31

PAR-1 is required for morphogenesis of the vulva
Daryl D. Hurd, Kenneth J. Kemphues

32

Dual role of the E/Daughterless protein HLH-2 with respect to the AC/VU decision
Xantha Karp, Iva Greenwald

33

Effects of Hyperactivation of EGL-15 on Sex Muscle Development
Isaac E. Sasson, Michael J. Stern

34

Heparan sulfate side chain modifying enzymes have important roles in neuronal development
Hannes Bülow, Oliver Hobert

35

Functions of the septins in C. elegans development
Fern P. Finger, John G. White

36

A GENETIC PATHWAY FOR THE CONTROL OF THE SEXUALLY DIMORPHIC DEATHS OF THE CEM NEURONS
Hillel Schwartz, Bob Horvitz

Saturday PM 4:45-6:50 NEUROBIOLOGY

Chair, Joe Dent

37

A novel gene sym-1 regulates synaptic differentiation in C.elegans
Edward H. Liao, Mei Zhen

38

Calcium/diacylglycerol-activated protein kinase C is essential for normal thermotaxis and responses to odorants.
Marianne Land, Charles S. Rubin

39

CaMKI AND THERMOSENSATION
John S. Satterlee, Piali Sengupta

40

Serotonin modulation of a chemosensory circuit in C. elegans
Michael Y. Chao, Hidetoshi Komatsu, Rhonda Hyde, Heather M. Dionne, Anne C. Hart

41

A Genetic Screen for G Protein Signaling Components, Neurotransmitters, and Neurons that Inhibit Egg-Laying Behavior in C. elegans.
I. Amy Bany, Michael Koelle

42

In vivo imaging of neuronal responses to touch and taste.
Rex Kerr, Hiroshi Suzuki, Massimo Hilliard, Christian Frokjar-Jensen, Dan Sloan, Jian Xue, Monica Driscoll, William Schafer

43

Dopamine signals through the G protein Galphao in C. elegans
Daniel L. Chase, Michael R. Koelle

44

Mechanosensory Channel Complex Interactions with the Extracellular Matrix
Lesley Emtage, Martin Chalfie

45

Cellular physiology of cultured C. elegans mechanosensory neurons
Laura Bianchi, Monica Driscoll

46

MEC-2 interacts with MEC-4 via stomatin-like region
Shifang Zhang, Martin Chalfie

47

THE PUTATIVE REGULATORY SUBUNITS SUP-10 AND SUP-18 REGULATE THE SUP-9 TWO-PORE POTASSIUM CHANNEL THROUGH A MECHANISM THAT IS DISTINCT FROM THAT OF THE UNC-93 SUBUNIT
Ignacio Perez de la Cruz, Bob Horvitz

48

Characterization of GLC-4: a novel glutamate-gated chloride channel subunit
Mark Pellegrino, Joseph Dent

49

The role of UNC-103, an ERG K channel, in electrical excitability in C. elegans
Christina I. Petersen, Svetlana Z. Stepanovic, Toni R. McFarland, Jeffrey R. Balser

Sunday AM 8:30-12:10 CELL BIOLOGY

Chair, Valerie Reinke

50

An Eph Receptor Sperm-Sensing Control Mechanism for Oocyte Meiotic Maturation
Michael A. Miller, Paul J. Ruest, Mary Kosinski, Steven K. Hanks, David Greenstein

51

Tubulin and centrosome rotation in the early C. elegans embryo
Amanda J. Wright, Craig P. Hunter

52

AN UNUSUAL MUTATION IN CDK-1 REVEALS THAT THE MAJOR EMBRYONIC CYCLIN DEPENDENT KINASE HAS A ROLE IN THE REGULATION OF CELL POLARITY
Martha C. Soto, Craig C. Mello

53

LIN-5 and GoLoco domain proteins function in a complex to regulate spindle positioning in the early embryo
Dayalan Srinivasan, Ridgely Fisk, Huihong Xu, Sander van den Heuvel

54

Microarray Analysis of MAP Kinase Signaling in the Germline of C. elegans
Stefanie West, Stuart K. Kim, Valerie Reinke

55

Calcineurin, a component of G-protein coupled phosphorylation pathways, is involved in movement, fertility, egg laying, and growth in C. elegans
Joohong Ahnn, Jungsoo Lee, Jiyeon Lee, Jaya Bandyopadhyay

56

Hypodermal expression of sma-3 is essential for body length regulation in C. elegans
Jianjun Wang, Rafal Tokarz, Cathy Savage-Dunn

57

Defining new components of TGF-beta signaling pathways in C. elegans.
Cole M. Zimmerman, Cathy Savage-Dunn, Lisa L. Maduzia, Andrew F. Roberts, Richard W. Padgett

58

spr-1, a suppressor of presenilin, encodes a conserved transcriptional repressor that may play a general role in LIN-12/Notch signalling
Sophie JARRIAULT, Iva GREENWALD

BREAK (30 minutes)

59

CED-13, a novel BH3 domain protein, regulates radiation-induced and developmental programmed cell death in C. elegans.
Shai Shaham, Nicole Wittenburg, Bjorn Schumacher, Anton Gartner, Simon Tuck, Barbara Conradt

60

UNC-98 is a Zn finger protein, which localizes to muscle cell nuclei and focal adhesion structures, and is required for myofibril organization
Kristie B. Mercer, Denise B. Flaherty, Hiroshi Qadota, Rachel K. Miller, Tina L. Tinley, Don G. Moerman, Guy M. Benian

61

Phosphorylation of the carboxy-terminus of ICP-1 by AIR-2 kinase stimulates AIR-2 kinase activity
John D. Bishop, Jill M. Schumacher

62

mat-1 Encodes the Anaphase Promoting Complex Sub-unit, CDC27
Penny Sadler, Jill Schumacher, Maziar Abdolrasulnia, Diane Shakes, Andy Golden

63

CUL-2 regulates the level of multiple cellular proteins
Vikas Dhingra, Edward T. Kipreos

64

Ubiquitin ligase CUL-2 is required for segregation of meiosis II sister chromatids and for establishing polarity
Ji Liu, Srividya Vasudevan, Edward Kipreos

65

RME-6, A New Regulator of Early Endocytic Events
Barth D. Grant

66

A critical role for mitochondria in C. elegans lifespan regulation
Siu Sylvia Lee, Raymond Lee, Julie Ahringer, Andrew Fraser, Ravi Kamath, Gary Ruvkun

67

clk-1 and ubiquinone biosynthesis
Abdelmadjid K. Hihi, Siegfried Hekimi

68

Analysis of telomerase in C. elegans
Bettina Meier, Yan Liu, Jonathan Hodgkin, Shawn Ahmed

Posters

69

Analysis of linker cell death
Mary C Abraham, Shai Shaham

70

Functional Analysis of PAR-6
Donato Aceto, Aaron J. Schetter, Kenneth J. Kemphues

71

FUNCTIONAL ANALYSIS OF THE MICRORNA GENES OF C elegans
Ezequiel A Alvarez-Saavedra, Eric A Miska, Allison L Abbott, Nelson C Lau, David P Bartel, Victor Ambros, Bob Horvitz

72

IDENTIFICATION AND CHARACTERIZATION OF SYNMUV SUPPRESSORS, INCLUDING A HOMOLOG OF THE CHROMATIN-REMODELING ATPASE ISWI
Erik C. Andersen, Xiaowei Lu, Scott Clark, Bob Horvitz

73

A DOPAMINE RECEPTOR MODULATING MECHANOSENSORY RESPONSES IN C.elegans.
Suparna Sanyal, Scott M. Summers, Rokhand Arvan, Richard F. Wintle, Eve Bigras, Dave Merz, Terence Hebert, William R. Schafer, Joseph G. Culotti, Hubert H. M. Van Tol

74

Nematode embryonic cell lineages are computationally efficient
Ricardo B. R. Azevedo, Markus Gumbel, Volker Braun, Paul-Michael Agapow, Wouter Houthoofd, Kim Jacobsen, Ute Platzer, Hans-Peter Meinzer, Gaëtan Borgonie, Armand M. Leroi

75

Potential Roles of CBP, CREB, and HDAC in Polyglutamine Induced Neurodegeneration in C. elegans
Emily Bates, Cindy Voisine, Martin Victor, Yang Shi, Anne C. Hart

76

Composition and Dynamics of the C. elegans Early Embryonic Transcriptome
Ryan Baugh, Andrew Hill, Donna Slonim, Gene Brown, Craig P. Hunter

77

SRC-1 acts in parallel with Wnt signaling to specify endoderm and to control cleavage orientation in early C. elegans embryos.
Yanxia Bei, Jennifer Hogan, Laura A. Berkowitz, Martha Soto, Christian E. Rocheleau, Kaming Pang, John Collins, Craig C. Mello

78

The C. elegans maternal-effect gene clk-2 is essential for embryonic development, encodes a protein homologous to yeast Tel2p and affects telomere length.
Claire Benard, Brent McCright, Yue Zhang, Stephanie Felkai, Bernard Lakowski, Siegfried Hekimi

79

MAU-2: a novel eukaryotic protein required for the guidance of cellular and axonal migrations along both body axes in Caenorhabditis elegans.
Claire Benard, Siegfried Hekimi

80

Towards the molecular identification of srf-9, srf-8 and srf-4
Kathrin A. Belanger, Patricia Berninsone

81

exc Mutants Suppress kal-1 Induced Axonal Branching and Misrouting
Katherine L. Berry, Hannes E. Bülow, Oliver Hobert

82

CHARACTERIZATION OF lin-54 AND OTHER GENES THAT NEGATIVELY REGULATE let-60 Ras SIGNALING DURING VULVAL DEVELOPMENT
Craig Ceol, Bob Horvitz

83

RNAi based synthetic screens in C. elegans
Julian Ceron, John Koreth, Peilin Tan, Jean Francois Rual, Marc Vidal, Anton Gartner, William G. Kaelin, Jr, Stuart H. Orkin, Sander van den Heuvel

84

Transcriptional repression in the early C.elegans embryo by HDA-1
Julián Cerón, Pascale Dufourcq, Junko Obata, Valerie Reinke, Yang Shi

85

Functional analysis of AMPA-type glutamate receptor cytosolic tail sequences and their contribution to receptor localization at synapses
Howard Chang, Christopher Rongo

86

Identification and Analysis of mutations affecting fertilization in C. elegans
Indrani Chatterjee, Emily Putiri, Pavan Kadandale, Andrew Singson

87

Identification and Analysis of mutations affecting fertilization in C. elegans.
Indrani Chatterjee, Emily Putiri, Pavan Kadandale, Andrew Singson

88

Biochemical and genetic analysis of RNA interference in C. elegans
Chun-Chieh Chen, Darryl Conte, Hiroaki Tabara, Craig Mello

89

A genetic screen to isolate suppressors of a temperature-sensitive mutation of the separase gene in C. elegans.
Barry A. Chestnut, Andy Golden

90

Molecular Studies and Electrophysiological Characterization of Cyclic Nucleotide Gated Channels in Caenorhabditis elegans
SukWoo Cho, Kyu-Yeong Choi, Byung-Jae Park, Chul-Seung Park, Joo Hong Ahnn

91

Use of CeHR Axenic Medium for Exposure and Gene Expression Studies
Eric D. Clegg, Hugh F. LaPenotiere, Deborah Y. French, Maria Szilagyi

92

A possible role for the DNA damage checkpoint in aging
Iuval Clejan, Arno Alpi, Anton Gartner, Shawn Ahmed

93

Finding Players in the CeTwist Pathway
Peng Wang, Ann K. Corsi

94

Functional analysis of the him-3 gene family
Florence Couteau, Monique C. Zetka

95

Effects of mutations in C. elegans beta integrin cytoplasmic tyrosine residues
Erin J. Cram, Myeongwoo Lee, Jean Schwarzbauer

96

LOCALIZATION DYNAMICS OF THE A/P POLARITY MACHINERY IN THE 1-CELL EMBRYO.
Adrian A Cuenca, Aaron Schetter, Donato Aceto, Ken Kemphues, Geraldine Seydoux

97

Ray pattern and P3.p cell lineage evolution in Caenorhabditis.
Cynthia R. Davidson, Justin C. Bohrer, Scott E. Baird

98

Anaphase Promoting Complex function during meiosis in Caenorhabditis elegans.
Edward S. Davis, Barry A. Chestnut, Andy Golden

99

Cell fusion in C. elegans: Function of a novel protein EFF-1.
Jacob J. del Campo, William A. Mohler

100

A predicted innexin, LEP-1, is required for male tail tip morphogenesis in C. elegans
Tania Del Rio-Albrechtsen, Ying Yang, David H.A. Fitch

101

Role of the RBCC-NHL genes in C. elegans temporal development
Ilana DeLuca, Betsy Maller, Frank Slack

102

Genetic Analysis of Abl in Regulating C. elegans Germ cell Apoptosis
Xinzhu Deng, Alberto Villanueva, Randy Hofmann, Megan Dell, Oliver Hobert, Zvi Fuks, Gian Garriga, Michael O. Hengartner, Richard N. Kolesnick

103

Reversal Behavior in C.elegans
Beibei Zhao, Joseph A. Dent

104

Poly(ADP-ribose) polymerases and poly(ADP-ribose) glycohydrolases in C. elegans: molecular characterization of the pme gene family members.
Serge Desnoyers, Catherine Gravel, Steve N. Gagnon

105

Vacuolar-type H+-ATPase E subunit is required for embryogenesis and yolk transfer in C. elegans.
Bijaya Kumar Dhakal, Kyu Yeong Choi, Yon Ju Ji, Jae-Ran Yu, Joohong Ahnn

106

Functional Study of Caenorhabditis elegans Cadherins
Yukan Duan, Edward M Hedgecock

107

Does heritable interference induced by dsRNA involve transcriptional silencing in C. elegans?
Thomas F. Duchaine, Darryl Conte Jr., Craig C. Mello

108

Reconstruction of connectivity in the C. elegans male nervous system
Scott W. Emmons, David H. Hall, Michael Y. Zhang, Haftan Eckholdt

109

Towards the determination of the structure of the MEC-4 amino terminal intracellular domain
John K. Everett, Guy Montelione, Monica Driscoll

110

Investigation of CUL-2 Complex Components
Hui Feng, Edward T. Kipreos

111

Investigation of CUL-2 Complex Components
Hui Feng, Edward T Kipreos

112

Check out my Profile! Isolation of mutants with altered str-1 expression levels using automated, high-sensitivity fluorescence profiling
Anthony A. Ferrante, Britta Moellers, Vance Chang, Bruce Holcombe, Steve Alam

113

dre-1, a heterochronic gene affecting gonadal and extragonadal developmental age
Nicole Fielenbach, Adam Antebi

114

The role of LIN-5 and GoLoco domain proteins in spindle position determination
Ridgely Fisk, Dayalan Srinivasan, Huihong Xul, Sander van den Heuvel

115

Nematode titins: giant polypeptides composed of multiple repeating motifs expressed in muscle
Denise B. Flaherty, Kim M. Gernert, Nataliya Shmeleva, Mark Borodovsky, Xuexin Tang, Kristina B. Mercer, Shoichiro Ono, Guy M. Benian

116

Identification of Genes Specifically Required for Ventralward Axon Guidance in C. elegans
Kazuko Fujisawa, Joe Culotti

117

TWO MODIFIER SCREENS FOR NEW GENES INVOLVED IN PROGRAMMED CELL DEATH
Brendan D. Galvin, Peter W. Reddien, Bob Horvitz

118

One Goal - three Approaches: Exploring Genetics of Necrotic-like Cell Death in C. elegans
Beate Gerstbrein, Keli Xu, Vienna Lo, Chasmar Schreiber, Guannan Wang, Monica Driscoll

119

Attempts to understand Semaphorins signaling pathway in C.elegans.
Val E. Ginzburg, Joseph G. Culotti

120

NHR-6: a nuclear receptor transcription factor required for ovulation.
Chris R. Gissendanner, Jianping Xiao, Tri Q. Nguyen, Marius Hoener, Ann E. Sluder, Claude V. Maina

121

mig-14 functions in multiple developmental processes regulated by Wnt signaling
Julie E. Gleason, David M. Eisenmann

122

Characterization of lon-2 and its suppressors
Tina L. Gumienny, Lisa Maduzia, Cole Zimmerman, Andrew Roberts, Huang Wang, Richard W. Padgett

123

Wormatlas: A web-based behavioral and structural atlas of C. elegans
David H. Hall, Thomas Boulin, Maurice Volaski, Michael Zhang, Constantinos Polydorou, Zeynep F. Altun

124

Mapping and characterization of the germ line desilencing mutation sig-7
David Han, Zhorab Najarian, Bill Kelly

125

Identification of Loci Required for the Establishment of Anterior/Posterior Polarity in C. elegans
Yingsong Hao, Geraldine Seydoux

126

FUNCTIONAL STUDIES OF THE CLASS B SYNMUVS, GENES REQUIRED FOR NEGATIVE REGULATION OF VULVAL INDUCTION, AND CHARACTERIZATION OF THE CLASS B SYNMUV GENE lin-61
Melissa Harrison, Xiaowei Lu, Bob Horvitz

127

Two vacuolar ATPase B subunits exist in the nematode genome, and spe-5 encodes one that is required for spermatogenesis
Paul D. Hartley, Steven W. L'Hernault

128

Cathepsin Z is expressed in major hypodermal cells of C elegans and has a role in worm's molting/development
Sarwar Hashmi, Jun Zhang, Sara Lustigman

129

A SCREEN FOR ESSENTIAL CELL-DEATH GENES
Anna Heineman, Bob Horvitz

130

Characterization of the 12 C. elegans Regulator of G Protein Signaling (RGS) Proteins
Heather A. Hess, Michael R. Koelle

131

A mod-5 SUPPRESSION SCREEN FOR GENES INVOLVED IN SEROTONERGIC NEUROTRANSMISSION
Megan Higginbotham, Rajesh Ranganathan, Bob Horvitz

132

Expression and localization of UNC-13 proteins in C. elegans
Kelly Howell, Kristen Servent, Judith Lytle, Lynn Schwarting, Rebecca E. Kohn

133

The enhancer-of-akt-1 genes eak-1 through eak-5 may define novel components of DAF-2/insulin-like signaling
Patrick J. Hu, Gary B. Ruvkun

134

The putative RNA-binding proteins MEX-5, MEX-6, and SPN-4 regulate MEX-3 localization and activity to control PAL-1 spatial patterning
Nancy N. Huang, Craig P. Hunter

135

FGF Regulation of Fluid Balance in C. elegans
Peng Huang, Michael J. Stern

136

A SCREEN FOR GENES SYNTHETICALLY LETHAL WITH lin-35 Rb
Mike Hurwitz, Bob Horvitz

137

Genetic mapping and Characterization of Bisphenol-A Resistant Mutants in Caenorhabditis elegans.
Yonju Ji, Hyun-ok Song, Joohong Ahnn

138

Establishment and maintenance of directional left/right asymmetry in the bilateral taste receptor neurons ASEL and ASER
Robert J. Johnston Jr., Sarah Chang, Oliver Hobert

139

Identifying the downstream effector(s) of GOA-1, the C. elegans ortholog of the major human brain G alpha protein.
Antony M Jose, Michael R Koelle

140

pvl-5 maintains hypodermal cell identity by preventing apoptosis during C. elegans vulval development.
Pradeep M. Joshi, David M. Eisenmann

141

Characterization of Genes Required for Fertilization in C. elegans
Pavan Kadandale, Brian Geldziler, Andrew Singson

142

Characterization of Genes Required for Fertilization in C. elegans
Pavan Kadandale, Brian Geldziler, Andrew Singson

143

Identification of a new PHA-4 DNA binding sequence by site selection.
John M. Kalb

144

Formal Modeling, Simulation and Analysis of C. elegans Development
Na'aman Kam, Rami Marelly, Hillel Kugler, Irun R. Cohen, Amir Pnueli, Michael J. Stern, E. Jane Albert Hubbard, David Harel

145

Roles of PP2A and PAR-1 in vulval development.
Gautam Kao, Meera V. Sundaram

146

Identification and characterization of enhanced RNAi mutants
Scott Kennedy, Gary Ruvkun

147

A large scale screen for dpy mutants in Pristionchus pacificus
Charlotte C.S. Kenning, Isabel Kipping, Alexandra Brand, Waltraud Sinz, Hanh Witte, Ralf J. Sommer

148

Regulation of Early Germline Proliferation in C. elegans
Darrell J. Killian, Aisha Chaudhary, Dan Culliford, E. Jane Albert Hubbard

149

The interactors of CKI-2 suggest a novel CDK inhibitor regulatory mechanism in C. elegans
Dae Young Kim, Richard Roy

150

Evolutionary changes in stoma ultrastructure within the nematode taxon Diplogastridae
Karin Kiontke, Shu-Yi Chiou, David H. A. Fitch, Alexander Fürst v. Lieven, Walter Sudhaus

151

REGULATION OF C. ELEGANS MALE MATE-SEARCHING BY MONOAMINE NEUROTRANSMITTERS AND SIGNALS FROM THE GONAD
Jonathan Lipton, Gunnar Kleemann, Michelle Lenzi, Scott W. Emmons

152

The Golgi P-type Ca2+ ATPase pump PMR1 regulates calcium homeostasis in C.elegans
Kyungmin Ko, Jeonghoon Cho, Joohong Ahnn

153

A screen for suppressors of rod-like lethality of lin-1 eor-1 double mutants
Kyunghee Koh, Meera Sundaram

154

Molecular localization of nT1 breakpoints and the role of EGL-18 and ELT-6 GATA factors in vulval development
Kyunghee Koh, Yelena Bernstein, Meera Sundaram

155

Phenotypic characterization and initial mapping of spe-42(eb5), a new fertilization defective mutant
Tim L. Kroft, Steven W. L'Hernault

156

Cell cycle dependent localization of the C. elegans Myt1 ortholog wee-1.3
Samuel T. Lamitina, Steven W. L'Hernault

157

The OTX-related homeodomain protein CEH-37 is required for the development of the AWB olfactory neurons.
Anne Lanjuin, Julia K. Thompson, Piali Sengupta

158

Bioinformatic analysis of the leucine-rich repeat protein family in Caenorhabditis elegans
Ji Young Lee, Edward M. Hedgecock

159

Programmed cell death in Pristionchus pacificus
Kwang-Zin Lee, Ralf J. Sommer

160

Molecular cloning of an embryonic lethal gene (jh1) on chromosome I in C. elegans
Wonhae Lee, Joohong Ahnn

161

Genome-wide RNAi screen for genes that control germ cell apoptosis in the nematode C. elegans
Guillaume Lettre, Ekaterini Kritikou, Martin Jaeggi, Andrea Calixto, Andrew Fraser, Ravi Kamath, Julie Ahringer, Michael O. Hengartner

162

Suppressor screen of the UNC-5 induced precocious migration of the hermaphrodite distal tip cells.
Naomi Levy-Strumpf, Lijia Zhang, Joseph G. Culotti

163

sma-9 functions in TGF-beta /Sma/Mab pathway and encodes a zinc finger protein homologous to Drosophila Schnurri
Jun Liang, Ling Yu, Cathy Savage-Dunn

164

hbl-1, a homologue of Drosophila hunchback, regulates temporal patterning during C. elegans post-embryonic development
Shin-Yi Lin, Mary Abraham, Eun-Young Choi, Steven Johnson, Amy Pasquinelli, Gary Ruvkun, Frank Slack

165

Characterization of the Molecular Determinants of EGL-15 FGFR Signaling Specificity
Te-Wen Lo, Jay Goodman, Michael Stern

166

EGL-26 Functions Involved in Vulva Morphogenesis
Andrew Ma, Rita Sharma, Wendy Hanna-Rose

167

Regulation of body size by lon-1 and kin-29 downstream of the dbl-1 signaling pathway
Lisa L. Maduzia, Andrew F. Roberts, Huang Wang, Stephen Cohen, Richard W. Padgett

168

ego-2 FUNCTIONS IN GLP-1-MEDIATED SIGNALING IN THE GERM LINE AND EMBRYO.
Eleanor Maine, Deb Springer, Dave Hansen, Tim Schedl

169

Regulation and function of DAF-9 cytochrome P450 in the dauer pathway
Ho Yi Mak, Gary Ruvkun

170

Regulation and function of DAF-9 cytochrome P450 in the dauer pathway
Ho Yi Mak, Gary Ruvkun

171

Analysis of Glutamatergic Neurotransmission by Knockout of Glutamate Transporter Genes.
Itzhak Mano, Tricia Wright, Monica Driscoll

172

The Role of Phosphorylation by PKC-3 in PAR-3 Function
Melissa A. Mason, Kenneth J. Kemphues

173

Evolution and fuction of the secretin family of serpentine receptors
Surjeet Mastwal, David Yu, Edward Hedgecock

174

Using RNAi to identify regulators of neural differentiation gene pag-3
Joan B. McDermott, Michael W. Krause

175

The role of C. elegans autophagy genes in development
Alicia Meléndez, David H. Hall, Beth Levine

176

The paired-like homeodomain protein SNS-10 is required for the development of the AWA olfactory neurons
Tali Melkman, Piali Sengupta

177

STEROL REQUIREMENTS AND DISTRIBUTION IN npc-1 NULL C. ELEGANS
Mark Merris, William G. Wadsworth, John Lenard

178

Post-embryonic Developmental Expression Chronograms: a new functional genomics data type generated using a nematode fluorescence sorting system
William A. Mohler, Rock Pulak, Anthony Ferrante

179

EGL-47 is a putative G protein-coupled receptor found in the synaptic termini of HSN neurons that stimulate egg-laying behavior in C. elegans
James Moresco, Michael Koelle

180

Isolation and characterization of the cks-1 mutant in C. elegans
Kuniaki Nakamura, Soyoung Kim, Craig C. Mello

181

Characterizing bar-1 function and regulation during C. elegans vulval development
Lakshmi Natarajan, David M. Eisenmann

182

cgh-1, a conserved germline predicted RNA helicase required for gametogenesis and oocyte survival in C. elegans
Rosa E. Navarro, T. Keith Blackwell

183

Mechanism of cog-2 function
Brian L. Nelms, Wendy Hanna-Rose

184

Cell Fusion Defects in eff-1 Adults Seen by TEM
Ken Nguyen, Irina Kolotuev, Benjamin Podbilewicz, David H. Hall

185

Body size regulation in C. elegans -Progress in cloning lon-4, lon-5, lon-6 and lon-7.
Josefin Nyström, Anthony Flemming, Richard W. Padgett, Armand Leroi, Simon Tuck

186

Identification of Effectors of zyg-1-Dependent Centrosome Duplication
Kevin F. O'Connell, Kevin R. Kopish

187

Functional Studies of the eff-1 Promoter
Eugene Opoku-Serebuoh, William A. Mohler

188

Developmental control of nuclear divisions in the C elegans intestine.
Jimmy Ouellet, Richard Roy

189

SERPIN FUNCTION IN C. ELEGANS: A GENETIC AND BIOCHEMICAL ANALYSIS
Stephen C. Pak, Cliff J. Luke, Yuko S. Askew, David J. Askew, David R. Mills, Christopher Tsu, Vasantha Kumar, Sule Cataltepe, Gary A. Silverman

190

Evolution of intron size in Caenorhabditis elegans
MF Palopoli, A Prachumwat

191

An N-terminal region of C. elegans RGS proteins EGL-10 and EAT-16 directs inhibition of Go alpha versus Gq alpha signaling
Georgia A. Patikoglou, Michael R. Koelle

192

Identification of functions for downstream targets of the duaer-regulating TGF-beta pathway in Caenorhabditis elegans
Manjing Pan, Garth I. Patterson

193

Computational identification of regulatory elements involved in TGFbeta-regulated dauer formation in Caenorhabditis elegans.
Garth I. Patterson, Shalin Jani

194

pom-1 is a Conserved Regulator of Cell Polarity and Cell Division in the C. elegans Embryo and Fission Yeast
Jason Pellettieri, Valerie Reinke, Stuart Kim, Geraldine Seydoux

195

The Function of Tax-4 in Surface Antigen Switching in C. elegans
Samuel M. Politz, Jennifer A. Cormier

196

Computational Analysis of Putative Surface Protein Sequences with a Specific Six-Cysteine Pattern
Samuel M. Politz, Andrea Emery

197

Characterization and Evolutionary Relationship of src-1 and src-2
Chery A. Pugh, Matthias Mann, Akhilesh Pandey, John J. Collins

198

Sexual Transformation of Caenorhabditis hybrids.
Jason Puglise, Scott E. Baird

199

Multi-parameter & Dual Color Fluorescence Analysis and Flow Sorting of C. elegans.
Rock Pulak, Jen Kean, Britta Moellers

200

EXPRESSION OF nhr-111, AN APPARENT C. elegans-SPECIFIC NUCLEAR HORMONE RECEPTOR
Kristy Reinert, Bruce Wightman

201

Gene Regulation by TGF-beta Signaling In C. elegans.
Andrew F. Roberts, Shi-lan Wu, Kristin Checchio, Richard W. Padgett

202

Investigating the role of cnk-1 in Ras signaling
Christian E Rocheleau, Meera V Sundaram

203

Germinal Center Kinase (GCK-1) affects meiotic prophase progression in the Caenorhabditis elegans germline
Katherine Roeder, Jill Schumacher

204

Maternal-effect suppressors of the cdc-25.1(gf)-dependent cell cycle defects in the intestinal lineage of C. elegans.
John Drysdale, Ivana Kostic, Richard Roy

205

Phagocytosis of necrotic corpses--a new gene involved in NCD corpse disposal
D. Royal, M.A. Royal, I. Cislaru, H. Sharkey, M.Driscoll

206

Toward Understanding Mechanotransducing Channel Function: mec-6 Influences the Stability of the MEC-4 Subunit but Not the MEC-10 Subunit
D. Royal, N. Tavernarakis, M. Driscoll

207

Developmental control of cell cycle in C. elegans
R. Mako Saito, Sander van den Heuvel

208

Does the heterochronic gene lin-41 interact with the C. elegans homologues of the spatial patterning genes, nanos and pumilio?
Nazli Saka, Mona Carlson, Frank Slack

209

GENES REQUIRED FOR AMPA-TYPE GLUTAMATE RECEPTOR LOCALIZATION IN C. ELEGANS
Henry Schaefer, Christopher Rongo

210

Nematodes harboring a longevity gene mutation display a time-limited (mid-life) protection of the age-related deterioration of muscle cell nuclei
Peter Schmeissner, Yuko Sakano, Monica Driscoll

211

Preparation of Nematode, Bacteria, and Yeast Nucleic Acids and Proteins by Pressure Cycling Technology (PCT)
Richard Schumacher, Feng Tao, Nathan P. Lawrence, Chunqin Li, Wayne W. Miller, Mark M. Manak

212

A NOVEL PHENOTYPE OF TRANSGENE MISEXPRESSION YIELDS NEW INSIGHT INTO THE SYNMUV GENES
Hillel Schwartz, Bob Horvitz

213

Identifying new genes involved in TGFbeta signaling in C. elegans
Kamila A. Sekiewicz, Garth I. Patterson

214

daf-6, a gene regulating morphology of the glial-like amphid sheath cells, encodes a Patched-related protein.
Shai Shaham, Max Heiman, Mark Schroeder, Elliot Perens

215

Phenotypic characterization of dsc mutants
Yukimasa Shibata, Robyn Branicky, Siegfried Hekimi

216

A Gene targeting strategy using the yeast endonuclease I-SceI in C.elegans
Masaki Shirayama, Jennifer A. McCullough, Craig C. Mello

217

Mutator genes in Caenorhabditis elegans
Cristina Silvescu, Sarah Kenick, John J. Collins

218

Two homologues of rde-1, alg-1 and alg-2, are implicated in RNA interference and microRNA pathways.
Martin J. Simard, Erbay Yigit, Craig C. Mello

219

Cameleon-reported Insights into Touch Receptor Channel Function in Vivo
Rex Kerr, Hiroshi Suzuki, Dan Slone, Jian Xue, Monica Driscoll, William Schafer

220

A new isoform of UNC-89 with two MLCK-like protein kinase domains
Tracey M. Small, Denise B. Flaherty, Kristina B. Mercer, Teresa M. Williams, Mark Borodovsky, Guy M. Benian

221

COORDINATE REGULATION OF GLUTAMATE RECEPTORS AND ncs-1 IN INTERNEURONS BY fax-1 AND unc-42
Eric L. Smith, Rebecca Haviland, Rebecca Lombel, Sheila Mathieson, Bruce Wightman

222

TOWARD A MOLECULAR ANALYSIS OF ego-3, ego-4, AND ego-5
Elyse Connors, Deb Springer, Lance Hale, Larissa Wirlo, Eleanor Maine

223

Structure function analysis of glutamate-gated chloride channels
Tanja Starc, Joseph A. Dent

224

Alae Mutant Defects Assessed by SEM and TEM
Tylon Stephney, Carolyn Marks, Adam Hartley, Min Ren, Charles S. Rubin, David H. Hall

225

ASYMMETRIC PROTEIN LOCALIZATION IN ZYGOTES: A ROLE FOR PROTEIN DEGRADATION?
Michael Stitzel, Geraldine Seydoux

226

C. elegans G protein-coupled receptor kinase, grk-2, and chemosensation
Hana Sugimoto, Heather Dionne, Denise M. Ferkey, Anne C. Hart

227

daf-5, a TGF-beta signaling output controls stem cell division in dauer larvae
Li Sun, Karen Zimmerman, Garth I Patterson

228

F54C4.3 is an overexpression suppressor of mat-3/APC8
Meera Sundaram, Jeff Doto, David Garbe

229

Systemic RNAi: Form and Function
Marie Sutherlin, William Winston, Christina Molodowitch, Craig Hunter

230

A strategy for high-throughput gene mapping in Caenorhabiditis elegans.
Kathryn A. Swan, Damian E. Curtis, Kathleen B. McKusick, Alexander V. Voinov, Felipa A. Mapa, Michael R. Cancilla

231

STRUCTURAL DIFFERENCES IN THE GOLGI APPARATUSES OF WILD-TYPE AND unc-20 MUTANT ADULTS
Jessica Tanis, Peter Alff, Jeffrey Doto, David Hall, Elizabeth R. McCain, Bruce Wightman

232

Understanding the regulation of C. elegans Hox Gene egl-5 through dissection of cis-regulatory elements
Yingqi Teng, Scott W. Emmons

233

FUNCTIONAL GENOMIC ANALYSIS OF SIGNALING PATHWAYS INVOLVED IN C. ELEGANS DAUER FORMATION
Muneesh Tewari, Patrick Hu, Svetlana Busiguina, Sabrina Chaklos, Jin Sook Ahn, Gary Ruvkun, Marc Vidal

234

A new genetic screen to identify genes that regulate life span
Heidi A. Tissenbaum, Christian Grove, Nenad Svrzikapa

235

A large-scale screen for temperature sensitive embryonic lethal mutants.
Chris Trzepacz, Masaki Shirayama, Ka Ming Pang, Kuniaki Nakamura, Takao Ishidate, Dan Brownell, Craig C. Mello

236

IDENTIFICATION OF LOCALIZATION DOMAINS OF UNC-43
Toru Umemura, Christopher Rongo

237

MOLECULAR AND PHENOTYPIC CHARACTERIZATION OF A CAENORHABDITIS ELEGANS MUTANT WITH ENHANCED SUSCEPTIBILITY TO PSEUDOMONAS AERUGINOSA
Jacinto M. Villanueva, Frederick M. Ausubel

238

Genetic and molecular analysis of polyglutamine neurotoxicity in C elegans
Cindy Voisine, Peter W. Faber, Emily A. Bates, Daphne C. King, Gregory A. Burkett, Anne C. Hart

239

EGO-1 EXPRESSION
Valarie Vought, Deb Springer, Eleanor Maine

240

Regulation of Programmed Cell Death in C. elegans Tail Spike Cells
Carine L.M. Waase, Shai Shaham

241

Mechanism of lin-39 regulation by the Wnt and Ras pathways during vulval devemlopment
Javier Wagmaister, David Eisenmann

242

Confocal and whole-genome-microarray analysis of STAT ortholog deletion mutant in C. elegans
Yaming Wang, David E. Levy

243

Screens to identify longevity-control genes in C. elegans
Mark A. Wilson, Catherine A. Wolkow

244

Regulation of Touch Cell Functional Genes
Ji Wu, Martin Chalfie

245

C. elegans DAZ-1 is necessary in early prophase of female meiosis
Rika Maruyama, Sachiko Endo, Asako Sugimoto, Masayuki Yamamoto

246

mig-15 acts with rac to control the orientation of vulval cell divisions and migrations in C.elegans
ZaiFang Yu, Ranjana S.Kishore, Meera V. Sundaram

247

Identification and characterisation of novel genes involved in the engulfment of apoptotic cell corpses in C. elegans
Stephanie Zuellig, Michael O. Hengartner


The author index follows the abstracts.