pCHNFI-A2 up to 3 cutters Sequence Range: 1 to 5499 >SphI | >EcoRI >BspMI | >Sse8387I | | | | | | >start_of_CMV_promoter/enhancer | | | | | | | | | | 60 GAATTCGAGC TTGCATGCCT GCAGGTCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC 120 TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG 180 CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG >NdeI | | 240 GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA 300 TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC >BsaA1 >StyI | | >SnaBI >NcoI | | | | 360 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG 420 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG 480 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA >SacI | >TATA_box_start_of_CMV_promoter | | | | 540 TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA >BpmI >BbsI | | >presumed_start_site_of_CMV_promoter | | | | | | | 600 GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGACAC >BspE1 >XbaI >XhoI | | | >end_of_CMV_promoter_XbaI_site >start_of_SV40_splice_region | | | | | | | | | | 660 CGGGACCGAT CCAGCCTCCG GACTCTAGAG GATCCGGTAC TCGAGGAACT GAAAAACCAG >HpaI >EcoO109I | | >start_of_16S_RNA_intron >SV40_deletion_site_BamHI_site | | | | | | | | 720 AAAGTTAACT GGTAAGTTTA GTCTTTTTGT CTTTTATTTC AGGTCCCGGA TCCGGTGGTG >StuI | >last_base_of_16S_RNA_intron || || 780 GTGCAAATCA AAGAACTGCT CCTCAGTGGA TGTTGCCTTT ACTTCTAGGC CTGTACGGAA >EagI | >SacII | >NotI | >end_of_SV40_sequence >NotI_cloning_site | | | | 840 GTGTTACTTC TGCTCTAAAA GCTGCGGAAT TGTACCCGCG GCCGCCAGCC ATGTATCCTT M Y P> __________> >BciVI >SfiI | | | | 900 ACGATGTACC AGACTATGCT GGGCCTCACG GGCCGATGTA TTCTCCGCTC TGTCTCACCC Y D V P D Y A G P H G P> _______HA EPITOPE TRANSLATION___a____> M Y S P L C L T> _____b___MNFI-A4___________> >XmnI | | 960 AGGATGAGTT TCATCCTTTC ATTGAAGCAC TTCTGCCCCA TGTCCGCGCC TTCGCCTACA Q D E F H P F I E A L L P H V R A F A Y> __________b__________b______MNFI-A4________b__________b__________> >BspMI | >Sse8387I >XmnI | | | | | | 1020 CATGGTTCAA CCTGCAGGCC CGAAAGCGGA AATACTTCAA AAAACATGAG AAGCGCATGT T W F N L Q A R K R K Y F K K H E K R M> __________b__________b______MNFI-A4________b__________b__________> >BpuI102I | | 1080 CGAAAGAAGA GGAGAGGGCC GTGAAGGATG AACTGCTAAG CGAGAAGCCC GAGGTCAAGC S K E E E R A V K D E L L S E K P E V K> __________b__________b______MNFI-A4________b__________b__________> >MscI >EcoRV | | | | 1140 AGAAGTGGGC TTCCCGACTT CTGGCCAAGT TACGGAAAGA TATCCGACCC GAGTACCGAG Q K W A S R L L A K L R K D I R P E Y R> __________b__________b______MNFI-A4________b__________b__________> >BclI | 1200 AGGATTTTGT TCTTACAGTT ACAGGGAAAA AACCTCCATG CTGTGTTCTT TCCAACCCTG E D F V L T V T G K K P P C C V L S N P> __________b__________b______MNFI-A4________b__________b__________> >EcoNI | | 1260 ATCAGAAAGG CAAGATGCGG AGAATTGACT GCCTCCGCCA GGCAGATAAA GTATGGAGGT D Q K G K M R R I D C L R Q A D K V W R> __________b__________b______MNFI-A4________b__________b__________> >Tth111I >ScaI | | | | 1320 TGGACCTCGT CATGGTGATC TTGTTCAAAG GTATTCCGCT GGAAAGTACT GATGGCGAGC L D L V M V I L F K G I P L E S T D G E> __________b__________b______MNFI-A4________b__________b__________> 1380 GCCTTGTAAA GAGTCCACAG TGCTCTAATC CAGGGCTCTG TGTCCAGCCC CATCACATAG R L V K S P Q C S N P G L C V Q P H H I> __________b__________b______MNFI-A4________b__________b__________> >BsrGI | | 1440 GGGTTTCTGT AAAGGAACTC GATTTATATT TGGCATACTT TGTACATGCA GCAGATTCAA G V S V K E L D L Y L A Y F V H A A D S> __________b__________b______MNFI-A4________b__________b__________> >PflMI | >XcmI| | | | 1500 GTCAATCTGA AAGTCCCAGC CAGCCAAGTG AAGCTGACAT TAAGGACCAG CCAGAAAATG S Q S E S P S Q P S E A D I K D Q P E N> __________b__________b______MNFI-A4________b__________b__________> >DrdI | | 1560 GACATTTGGG CTTCCAGGAC AGCTTTGTCA CATCAGGTGT TTTCAGTGTG ACTGAGCTAG G H L G F Q D S F V T S G V F S V T E L> __________b__________b______MNFI-A4________b__________b__________> 1620 TAAGAGTGTC ACAAACACCA ATAGCTGCAG GAACCGGCCC CAATTTTTCT CTCTCTGATT V R V S Q T P I A A G T G P N F S L S D> __________b__________b______MNFI-A4________b__________b__________> >BplI >BsrDI >BsaI | | | | | | 1680 TGGAAAGTTC TTCATACTAC AGCATGAGTC CAGGAGCAAT GAGGAGGTCT CTGCCCAGCA L E S S S Y Y S M S P G A M R R S L P S> __________b__________b______MNFI-A4________b__________b__________> >BplI | | 1740 CATCCTCTAC CAGCTCTACA AAGCGCCTCA AGTCTGTGGA GGATGAAATG GACAGTCCTG T S S T S S T K R L K S V E D E M D S P> __________b__________b______MNFI-A4________b__________b__________> >StyI >PflMI >BstXI | | | | | 1800 GTGAAGAACC ATTTTACACA GGCCAAGGGC GCTCCCCAGG GAGTGGCAGC CAGTCCAGTG G E E P F Y T G Q G R S P G S G S Q S S> __________b__________b______MNFI-A4________b__________b__________> >PflMI | | 1860 GATGGCATGA AGTAGAGCCA GGCTTGCCAT CTCCAAGCAC ACTGAAGAAG TCTGAGAAGT G W H E V E P G L P S P S T L K K S E K> __________b__________b______MNFI-A4________b__________b__________> >Eco57I >BsmI | | | | 1920 CTGGTTTCAG CAGCCCCTCC CCTTCGCAGA CCTCCTCCCT GGGAACAGCA TTCACACAGC S G F S S P S P S Q T S S L G T A F T Q> __________b__________b______MNFI-A4________b__________b__________> >EcoO109I | | 1980 ATCACCGACC TGTCATTACA GGACCCAGAG CAAGTCCACA TGCGACGCCA TCGACTCTCC H H R P V I T G P R A S P H A T P S T L> __________b__________b______MNFI-A4________b__________b__________> >BstXI | | 2040 ACTTTCCAAC GTCACCCATC ATCCAGCAGC CTGGGCCTTA CTTCTCACAC CCAGCCATCC H F P T S P I I Q Q P G P Y F S H P A I> __________b__________b______MNFI-A4________b__________b__________> >Bsu36I | | 2100 GTTACCACCC TCAGGAGACG CTGAAAGAGT TTGTCCAACT TGTCTGTCCT GATGCTGGTC R Y H P Q E T L K E F V Q L V C P D A G> __________b__________b______MNFI-A4________b__________b__________> 2160 AGCAAGCTGG ACAGGTGGGG TTCCTCAATC CCAATGGAAG CAGTCAAGGC AAGGTGCACA Q Q A G Q V G F L N P N G S S Q G K V H> __________b__________b______MNFI-A4________b__________b__________> >StuI | >MscI | | | | |2220 ACCCATTCCT CCCCACCCCA ATGTTGCCGC CGCCGCCACC ACCACCGATG GCCAGGCCTG N P F L P T P M L P P P P P P P M A R P> __________b__________b______MNFI-A4________b__________b__________> 2280 TGCCTCTGCC CATGCCAGAC ACCAAGCCTC CAACCACATC AACAGAAGGA GGTGCAGCCT V P L P M P D T K P P T T S T E G G A A> __________b__________b______MNFI-A4________b__________b__________> >BsgI >KpnI >BpmI | | | | | | 2340 CCCCCACCTC ACCGATCCTG GTACCTGGGA TAAAAGTTGC AGCATCCCAC CATCCTCCAG S P T S P I L V P G I K V A A S H H P P> __________b__________b______MNFI-A4________b__________b__________> 2400 ACAGACCACC TGACCCCTTC TCAACTCTGT AACATGGACG CAACCTCAAC CCGGCGCAGT D R P P D P F S T L *> __________b__MNFI-A4_b__________b__> 2460 TACAACTTCA CTGTCAGTGG AAGGGGAGCG AAACCAACTC GAATCAACTT ATACATGGAA 2520 ACAGCAAACA TCGTGGTCAA ACAGCAAAGG CCATAACTTT TTGGGATTCT TTTTTTTTCT 2580 TTTTTTCTTT TTTTCTTTTT TTTTTTCTTT TTTAATACTT TAGGGACTGT CGTAATTTCT >SacII | >start_of_SV40_polyA_region | | | 2640 CATGGTGCTG GAAATGGTTG GGCTGTGTGA CATTTGAAGT CTAGCTAGGG CCGCGGGGAT >BsmI | | 2700 CCAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA >PsiI | | 2760 AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC >BsmI | >HpaI >MunI | | | | | | | 2820 AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA GGGGGAGGTG >SphI | >AccI >BspMI |>HindIII | | | | >XbaI >SalI >Sse8387I | | | || | | | | >end_of_SV40_polyA_region | | | | | | || | | | | | | || | | | | 2880 TGGGAGGTTT TTTCGGATCC TCTAGAGTCG ACCTGCAGGC ATGCAAGCTT GGCGTAATCA 2940 TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA >VspI | | 3000 GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT >PvuII >VspI | | | | 3060 GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA >SapI | | 3120 ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC 3180 ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG >BspLU11I | >AflIII | >PciI | >AflIII | | 3240 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC 3300 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC >DrdI | | 3360 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA >BssSI | | 3420 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC >BciVI | | 3480 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT 3540 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG 3600 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC >AlwNI | | 3660 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA 3720 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT >Eco57I | | 3780 AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT 3840 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG 3900 CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG >RcaI | | 3960 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA >DraI >DraI | | | | 4020 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA 4080 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG >EamI105I | | 4140 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA >BsrDI | >BsaI >BpmI | | | | 4200 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG 4260 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT >VspI | | 4320 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT >Psp1406I | >FspI | >BsrDI | | | | | | 4380 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC 4440 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA >PvuI | | 4500 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT 4560 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC >ScaI | | 4620 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA >BcgI >Bcg1 | | | | 4680 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA >Psp1406I | >DraI >XmnI | | | | 4740 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA >Eco57I | >BssSI | | | | 4800 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT 4860 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC 4920 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA >BciVI | >SspI >RcaI | | | | | | 4980 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 5040 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC >RcaI >BssSI >EcoO109I | | | | | | 5100 TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT 5160 CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG >DrdI | | 5220 GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG 5280 GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA >BstAPI | >NdeI >NarI || | || 5340 GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG >FspI >PvuI | | | | 5400 CGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG >PvuII | | 5460 CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGT Sequence Range: 1 to 5499 Enzyme #Cuts Positions AccI 1 2848 AflIII 2 3224 3224 AlwNI 1 3640 BbsI 1 600 Bcg1 1 4656 BcgI 1 4622 BciVI 3 844 3427 4954 BclI 1 1199 BplI 2 1652 1684 BpmI 3 579 2320 4187 BpuI102I 1 1056 BsaA1 1 306 BsaI 2 1672 4178 BsgI 1 2293 BsmI 3 1908 2693 2786 BspE1 1 617 BspLU11I 1 3224 BspMI 3 12 979 2860 BsrDI 3 1663 4178 4352 BsrGI 1 1421 BssSI 3 3397 4781 5088 BstAPI 1 5289 BstXI 2 1797 2010 Bsu36I 1 2050 DraI 3 3983 4002 4694 DrdI 3 1524 3332 5201 EagI 1 819 EamI105I 1 4117 Eco57I 3 1863 3772 4784 EcoNI 1 1236 EcoO109I 3 702 1941 5093 EcoRI 1 1 EcoRV 1 1121 FspI 2 4339 5362 HindIII 1 2865 HpaI 2 666 2772 KpnI 1 2304 MscI 2 1104 2211 MunI 1 2781 NarI 1 5340 NcoI 1 326 NdeI 2 200 5288 NotI 1 819 PciI 1 3224 PflMI 3 1497 1782 1801 PsiI 1 2752 Psp1406I 2 4343 4716 PvuI 2 4487 5383 PvuII 2 3048 5412 RcaI 3 3944 4952 5057 SacI 1 534 SacII 2 819 2634 SalI 1 2847 SapI 1 3108 ScaI 2 1307 4597 SfiI 1 869 SnaBI 1 306 SphI 2 17 2863 Sse8387I 3 23 976 2857 SspI 1 4921 StuI 2 769 2216 StyI 2 326 1763 Tth111I 1 1266 VspI 3 2995 3054 4289 XbaI 2 624 2841 XcmI 1 1494 XhoI 1 640 XmnI 3 928 993 4716