pCHNFI-B up to 3 cutters Sequence Range: 1 to 5046 >EcoRI >SphI >Sse8387I | | | | | | 60 GAATTCGAGC TTGCATGCCT GCAGGTCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC 120 TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG 180 CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG >NdeI | | 240 GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA 300 TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC >SnaBI >NcoI | | >BsaA1 >StyI | | | | 360 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG 420 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG 480 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA >SacI | >BanII | >BanII | | 540 TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA >BsmB1 | | 600 GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGACAC >BspE1 >XbaI >XhoI | | | | | | 660 CGGGACCGAT CCAGCCTCCG GACTCTAGAG GATCCGGTAC TCGAGGAACT GAAAAACCAG >EcoO109I | >Psp5II | >HpaI >Psp5II | | | | 720 AAAGTTAACT GGTAAGTTTA GTCTTTTTGT CTTTTATTTC AGGTCCCGGA TCCGGTGGTG >BseRI >StuI | | | | 780 GTGCAAATCA AAGAACTGCT CCTCAGTGGA TGTTGCCTTT ACTTCTAGGC CTGTACGGAA >NotI | >EagI | >SacII | | 840 GTGTTACTTC TGCTCTAAAA GCTGCGGAAT TGTACCCGCG GCCGCCAGCC ATGTATCCTT M Y P> >SfiI | | 900 ACGATGTACC AGACTATGCT GGGCCTCACG GGCCGATGAT GTATTCTCCC ATCTGTCTCA Y D V P D Y A G P H G P M M Y S P I C L> >EcoRI >MunI | | | | 960 CTCAGGATGA ATTCCACCCA TTTATTGAGG CACTTCTTCC TCACGTCCGT GCAATTGCCT T Q D E F H P F I E A L L P H V R A I A> >Sse8387I >ScaI | | | | 1020 ATACTTGGTT CAACCTGCAG GCTCGAAAAC GCAAGTACTT TAAAAAGCAT GAGAAACGAA Y T W F N L Q A R K R K Y F K K H E K R> 1080 TGTCGAAGGA TGAAGAAAGG GCAGTCAAAG ACGAGCTGCT CAGTGAGAAG CCCGAAATCA M S K D E E R A V K D E L L S E K P E I> >MscI | >BstXI| >FspI >EcoRV | | | | | | | | 1140 AGCAGAAGTG GGCATCCAGG CTCCTGGCCA AACTGCGCAA AGATATCCGC CAGGAGTACC K Q K W A S R L L A K L R K D I R Q E Y> 1200 GGGAGGACTT TGTGCTTACC GTGACTGGCA AGAAGCACCC GTGCTGTGTC TTATCCAATC R E D F V L T V T G K K H P C C V L S N> >EcoNI >Tth111I | | | | 1260 CAGACCAGAA GGGTAAGATT AGGAGGATCG ACTGCCTGCG ACAGGCAGAC AAAGTCTGGC P D Q K G K I R R I D C L R Q A D K V W> >BplI | | 1320 GTCTGGATCT AGTCATGGTG ATCCTGTTCA AAGGCATCCC TTTGGAGAGT ACGGATGGAG R L D L V M V I L F K G I P L E S T D G> >BsgI >BplI >DraIII | | | | | | 1380 AGCGACTCAT GAAGTCCCCG CACTGCACAA ACCCAGCACT TTGTGTTCAG CCACACCACA E R L M K S P H C T N P A L C V Q P H H> >BsaA1 | | 1440 TCACAGTATC AGTTAAGGAG CTTGACTTGT TTTTGGCATA CTACGTGCAG GAGCAAGATT I T V S V K E L D L F L A Y Y V Q E Q D> >XhoI | >BsgI >KpnI | || | |1500 CTGGACAATC AGGAAGTCCA AGCCACAGTG ATCCTGCCAA GAATCCTCCA GGGTACCTCG S G Q S G S P S H S D P A K N P P G Y L> >BspE1 | | 1560 AGGACAGCTT TGTAAAATCC GGAGTCTTCA ATGTATCAGA GCTTGTGAGA GTATCCAGAA E D S F V K S G V F N V S E L V R V S R> >BstXI | | 1620 CACCCATAAC CCAGGGAACT GGAGTCAACT TCCCAATCGG AGAAATTCCC AGCCAACCAT T P I T Q G T G V N F P I G E I P S Q P> 1680 ACTATCATGA CATGAACTCT GGTGTGAACC TGCAGAGGTC GCTGTCTTCT CCACCGAGCA Y Y H D M N S G V N L Q R S L S S P P S> 1740 GCAAAAGACC CAAAACTATA TCTATAGATG AAAATATGGA GCCAAGTCCT ACAGGAGACT S K R P K T I S I D E N M E P S P T G D> >AlwNI | >PflMI | >PvuII | | | | 1800 TTTACCCCTC TCCAAATTCA CCAGCTGCTG GAAGTCGAAC ATGGCATGAA CGAGATCAAG F Y P S P N S P A A G S R T W H E R D Q> 1860 ATATGTCTTC TCCAACTACA ATGAAGAAGC CTGAGAAGCC ACTGTTTAGC TCTACATCTC D M S S P T T M K K P E K P L F S S T S> 1920 CACAGGATTC TTCCCCAAGA TTGAGCACTT TCCCCCAGCA CCATCATCCC GGAATACCTG P Q D S S P R L S T F P Q H H H P G I P> 1980 GAGTCGCGCA CAGTGTCATC TCAACTCGAA CTCCACCTCC GCCCTCACCG TTGCCATTTC G V A H S V I S T R T P P P P S P L P F> >EcoRV | >BseRI | | | | 2040 CGACGCAAGC TATCCTTCCT CCGGCACCTT CCAGCTACTT CTCTCATCCA ACAATCAGAT P T Q A I L P P A P S S Y F S H P T I R> >BsaA1 | >Bsu36I >SnaBI >Eco57I | | | | | | 2100 ATCCTCCTCA CCTGAATCCT CAGGATACTC TGAAGAACTA CGTACCTTCT TATGACCCAT Y P P H L N P Q D T L K N Y V P S Y D P> >KpnI >NheI >BglII | | | | | | 2160 CCAGTCCTCA AACGAGCCAG TCCTGGTACC TGGGCTAGCT TGGTTCCTTT TCAAGATCTA S S P Q T S Q S W Y L G *> >NheI | >NsiI| >SacII | | | | | | 2220 TGCATAGCTA GCTAGGGCCG CGGGGATCCA GACATGATAA GATACATTGA TGAGTTTGGA >BsmI | | 2280 CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT >HpaI >MunI >BsmI | | | | | | 2340 GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT >AccI | >XbaI >SalI | || | || 2400 TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT CGGATCCTCT AGAGTCGACC >HindIII | >Sse8387I >SphI | | | | | | 2460 TGCAGGCATG CAAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT >BsrBI | | 2520 CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC >VspI | | 2580 TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA >PvuII >VspI | | | | 2640 AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT >EarI | >SapI | | 2700 ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG >BsrBI | | 2760 CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC >AflIII | >AflIII | >BspLU11I | | 2820 GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG >DrdI | 2880 TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA 2940 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC >BssSI | | 3000 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC 3060 CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG >ApaLI | | 3120 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC 3180 TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA >AlwNI | | 3240 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG 3300 AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG >Eco57I | | 3360 AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT 3420 GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA 3480 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA 3540 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA 3600 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC 3660 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA >EamI105I | | 3720 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA >BsaI >BsrFI | | >BsrDI >BsrFI | | | | 3780 ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC >VspI | |3840 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT >Psp1406I | >FspI | >BsrDI | | | | | 3900 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC 3960 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT 4020 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC >PvuI | | 4080 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG 4140 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT >ScaI >BcgI | | | | 4200 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG >Bcg1 | | 4260 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA >Psp1406I | >XmnI | | 4320 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG >ApaLI | >Eco57I | >BssSI| | | | | 4380 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG 4440 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT >EarI >SspI | | | | 4500 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC >BsrBI | | 4560 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 4620 TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT >EcoO109I | >BssSI | >BsmB1 | | | | | | 4680 AAAAATAGGC GTATCACGAG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC >BsmB1 | | 4740 CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC >DrdI | | 4800 AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT >BstAPI | >ApaLI >NdeI | || | || 4860 GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA >NarI >FspI | | | | 4920 TGCGTAAGGA GAAAATACCG CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG >PvuI >EarI >PvuII | | | | | | 4980 GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT 5040 GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGT Sequence Range: 1 to 5046 Enzyme #Cuts Positions AccI 1 2395 AflIII 2 2771 2771 AlwNI 2 1767 3187 ApaLI 3 3085 4331 4828 BanII 2 534 534 Bcg1 1 4203 BcgI 1 4169 BglII 1 2154 BplI 2 1309 1341 BsaA1 3 306 1424 2081 BsaI 1 3725 BseRI 2 730 2035 BsgI 2 1328 1446 BsmB1 3 555 4654 4696 BsmI 2 2240 2333 BspE1 2 617 1518 BspLU11I 1 2771 BsrBI 3 2463 2704 4505 BsrDI 2 3725 3899 BsrFI 2 3744 3744 BssSI 3 2944 4328 4635 BstAPI 1 4836 BstXI 2 1103 1578 Bsu36I 1 2059 DraIII 1 1362 DrdI 2 2879 4748 EagI 1 819 EamI105I 1 3664 EarI 3 2655 4459 4947 Eco57I 3 2091 3319 4331 EcoNI 1 1239 EcoO109I 2 702 4640 EcoRI 2 1 909 EcoRV 2 1124 2040 FspI 3 1116 3886 4909 HindIII 1 2412 HpaI 2 666 2319 KpnI 2 1496 2129 MscI 1 1107 MunI 2 952 2328 NarI 1 4887 NcoI 1 326 NdeI 2 200 4835 NheI 2 2134 2167 NotI 1 819 NsiI 1 2164 PflMI 1 1767 Psp1406I 2 3890 4263 Psp5II 2 702 702 PvuI 2 4034 4930 PvuII 3 1764 2595 4959 SacI 1 534 SacII 2 819 2181 SalI 1 2394 SapI 1 2655 ScaI 2 996 4144 SfiI 1 869 SnaBI 2 306 2081 SphI 2 17 2410 Sse8387I 3 23 979 2404 SspI 1 4468 StuI 1 769 StyI 1 326 Tth111I 1 1251 VspI 3 2542 2601 3836 XbaI 2 624 2388 XhoI 2 640 1497 XmnI 1 4263