pCHNFI-C up to 3 cutters Sequence Range: 1 to 5110 >SphI | >EcoRI >BspMI | >Sse8387I | | | | | | | | 60 GAATTCGAGC TTGCATGCCT GCAGGTCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC 120 TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG 180 CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG >NdeI | | 240 GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA 300 TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC >BsaA1 >StyI | | >SnaBI >NcoI | | | | 360 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG 420 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG 480 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA >SacI | | 540 TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA >BsmB1 | | 600 GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGACAC >BspE1 >XbaI >XhoI | | | | | | 660 CGGGACCGAT CCAGCCTCCG GACTCTAGAG GATCCGGTAC TCGAGGAACT GAAAAACCAG >HpaI >EcoO109I | | | | 720 AAAGTTAACT GGTAAGTTTA GTCTTTTTGT CTTTTATTTC AGGTCCCGGA TCCGGTGGTG >BseRI >StuI | | | | 780 GTGCAAATCA AAGAACTGCT CCTCAGTGGA TGTTGCCTTT ACTTCTAGGC CTGTACGGAA >EagI | >NotI | >SacII | | 840 GTGTTACTTC TGCTCTAAAA GCTGCGGAAT TGTACCCGCG GCCGCCAGCC ATGTATCCTT M Y P> >SfiI >EcoNI | | | 900 ACGATGTACC AGACTATGCT GGGCCTCACG GGCCGATGTA TTCCTCCCCG CTCTGCCTGA Y D V P D Y A G P H G P M Y S S P L C L> >BssHII | | 960 CCCAGGATGA GTTCCACCCG TTCATCGAGG CGCTGCTGCC GCATGTGCGC GCCTTCGCCT T Q D E F H P F I E A L L P H V R A F A> >BspMI | >BssHII || >SexAI >Sse8387I >ScaI | | || | | | || | 1020 ACACCTGGTT CAACCTGCAG GCGCGCAAGC GCAAGTACTT CAAGAAGCAC GAGAAGCGCA Y T W F N L Q A R K R K Y F K K H E K R> >StyI >BssHII >BseRI | | | | | | 1080 TGTCCAAGGA CGAGGAGCGC GCGGTGAAGG ATGAGCTGCT GGGTGAAAAG GCCGAAGTGA M S K D E E R A V K D E L L G E K A E V> >MscI | | 1140 AGCAGAAGTG GGCGTCGCGG CTGCTGGCCA AGCTGCGCAA GGACATCCGG CCCGAGTGCC K Q K W A S R L L A K L R K D I R P E C> >NarI | | 1200 GTGAAGACTT CGTACTGGCT GTGACTGGCA AGAAGGCGCC AGGCTGTGTG CTGTCCAACC R E D F V L A V T G K K A P G C V L S N> >BstAPI >EcoNI | | | | 1260 CGGACCAGAA GGGCAAGATG CGGCGCATCG ACTGCCTGCG CCAGGCCGAC AAAGTGTGGC P D Q K G K M R R I D C L R Q A D K V W> >Tth111I | >SexAI >EcoNI | | | | | | 1320 GTCTGGACCT GGTCATGGTC ATCCTGTTCA AGGGCATCCC ACTGGAGAGC ACAGATGGTG R L D L V M V I L F K G I P L E S T D G> >BstAPI | >DraIII | | 1380 AGCGGCTGGT GAAGGCGGCT GCCTGTGCGC ACCCGGTGCT GTGTGTGCAG CCGCACCATA E R L V K A A A C A H P V L C V Q P H H> >BsgI | | 1440 TTGGCGTGGC CGTCAAGGAG CTGGACCTGT ACCTGGCCTA CTTTGTGCGG GAGCGCGATG I G V A V K E L D L Y L A Y F V R E R D> 1500 CGGAGCAGAG CAGCAGCCCC CGGACAGGTG TGGGCTCAGA CCAGGAGGAC AGCAAGCCCA A E Q S S S P R T G V G S D Q E D S K P> >BstXI | | 1560 TAACGCTGGA CACCACAGAC TTCCAGGAGA GCTTTGTCAC GTCAGGTGTG TTCAGTGTCA I T L D T T D F Q E S F V T S G V F S V> >BstEII >XcmI | | | |1620 CGGAGCTGAT CCAAGTGTCT CGGACACCTG TGGTGACCGG AACCGGACCC AACTTCTCAC T E L I Q V S R T P V V T G T G P N F S> >NgoAIV | >BstEII >NdeI >NaeI | | | | | | 1680 TGGGAGAGCT GCAGGGTCAC CTGGCATATG ACCTGAACCC GGCCAGCGCC GGCATGAGAA L G E L Q G H L A Y D L N P A S A G M R> >BseRI | | 1740 GGACTCTACC CAGCACCTCC TCCAGTGGGA GCAAGCGGCA CAAGTCGGGC TCGATGGAAG R T L P S T S S S G S K R H K S G S M E> 1800 AGGACGTGGA CACGAGCCCC GGCGGGGACT ACTACACGTC GCCCAACTCA CCCACGAGTA E D V D T S P G G D Y Y T S P N S P T S> 1860 GCAGCCGCAA CTGGACGGAA GACATAGAAG GAGGCATCTC ATCCCCGGTG AAGAAGACAG S S R N W T E D I E G G I S S P V K K T> 1920 AGATGGACAA ATCTCCATTC AACAGCCCGT CCCCCCAGGA CTCCCCCCGG CTCTCCAGCT E M D K S P F N S P S P Q D S P R L S S> >BsgI | 1980 TCACACAACA CCACAGGCCG GTCATCGCGG TACACAGTGG CATCGCGCGG AGCCCCCACC F T Q H H R P V I A V H S G I A R S P H> 2040 CGACCTCGGC TCTGCACTTC CCTGCCACGC CCATCCTGCC ACAGACAGCC TCCACCTACT P T S A L H F P A T P I L P Q T A S T Y> >EcoO109I | | 2100 TCCCCCATAC GGCCATCCGG TATCCGCCAC ATCTCAACCC CCAGGACCCG CTCAAGGATC F P H T A I R Y P P H L N P Q D P L K D> >BstXI | | 2160 TGGTGTCCCT GGCCTGTGAC CCGGCCACCC AGCAGCCTGG GCCGCCTGCT CTCCGCCCGA L V S L A C D P A T Q Q P G P P A L R P> >BglII | 2220 CACGTCCCCT GCAAACAGTC CCTTTGTGGG ATTAGGACGG AGGGATCCTC TTGCGGGAGA T R P L Q T V P L W D *> >NheI | >NsiI | >SacII | | | | | | 2280 TCTATGCATA GCTAGCTAGG GCCGCGGGGA TCCAGACATG ATAAGATACA TTGATGAGTT >BsmI | | 2340 TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC >BsmI | >HpaI >MunI | | | | | | 2400 TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT >AccI | >XbaI >SalI | || | 2460 TCATTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTCGGATC CTCTAGAGTC >HindIII | >BspMI | | | >Sse8387I |>SphI | | | | | | | | 2520 GACCTGCAGG CATGCAAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG 2580 TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG >VspI | | 2640 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC >TfiI | >PvuII >VspI | | | | | | | 2700 GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT >SapI | | 2760 GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT >TfiI | | 2820 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA >BspLU11I | | 2880 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 2940 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG >DrdI | | 3000 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 3060 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 3120 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT >ApaLI | | 3180 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 3240 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT >AlwNI | | 3300 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 3360 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT >Eco57I | | 3420 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 3480 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 3540 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG >RcaI >DraI | | | | 3600 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA >DraI | | 3660 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA 3720 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC >EamI105I | | 3780 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC >BsrDI | >BsaI | | 3840 TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC >VspI | 3900 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT >Psp1406I | | 3960 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT >BsrDI | | 4020 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC 4080 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG >PvuI | | 4140 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT 4200 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC >ScaI >BcgI | | | | 4260 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG >Bcg1 >DraI | | | | 4320 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT >XmnI | >Psp1406I | | 4380 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC >Eco57I | >ApaLI | | 4440 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC 4500 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA >SspI | | 4560 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG >RcaI | | 4620 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG >RcaI | | 4680 CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC >EcoO109I >BsmB1 | | | | 4740 CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA >BsmB1 | | 4800 AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG >DrdI | | 4860 GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA >BstAPI | >ApaLI >NdeI | || | || 4920 CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA >NarI | | 4980 CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGCCATTCG CCATTCAGGC TGCGCAACTG >PvuI >PvuII | | | | 5040 TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG 5100 TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT Sequence Range: 1 to 5110 Enzyme #Cuts Positions AccI 1 2459 AlwNI 1 3251 ApaLI 3 3149 4395 4892 Bcg1 1 4267 BcgI 1 4233 BglII 1 2218 BsaA1 1 306 BsaI 1 3789 BseRI 3 730 1047 1688 BsgI 2 1386 1977 BsmB1 3 555 4718 4760 BsmI 2 2304 2397 BspE1 1 617 BspLU11I 1 2835 BspMI 3 12 982 2471 BsrDI 2 3789 3963 BssHII 3 947 981 1037 BstAPI 3 1231 1355 4900 BstEII 2 1592 1635 BstXI 2 1505 2136 DraI 3 3594 3613 4305 DraIII 1 1355 DrdI 2 2943 4812 EagI 1 819 EamI105I 1 3728 Eco57I 2 3383 4395 EcoNI 3 900 1239 1287 EcoO109I 3 702 2084 4704 EcoRI 1 1 HindIII 1 2476 HpaI 2 666 2383 MscI 1 1107 MunI 1 2392 NaeI 1 1668 NarI 2 1176 4951 NcoI 1 326 NdeI 3 200 1646 4899 NgoAIV 1 1668 NheI 1 2231 NotI 1 819 NsiI 1 2228 Psp1406I 2 3954 4327 PvuI 2 4098 4994 PvuII 2 2659 5023 RcaI 3 3555 4563 4668 SacI 1 534 SacII 2 819 2245 SalI 1 2458 SapI 1 2719 ScaI 2 996 4208 SexAI 2 963 1267 SfiI 1 869 SnaBI 1 306 SphI 2 17 2474 Sse8387I 3 23 979 2468 SspI 1 4532 StuI 1 769 StyI 2 326 1024 TfiI 2 2670 2810 Tth111I 1 1269 VspI 3 2606 2665 3900 XbaI 2 624 2452 XcmI 1 1616 XhoI 1 640 XmnI 1 4327