pCHNFI-X up to 3 cutters Sequence Range: 1 to 4995 >SphI >PstI | | >EcoRI >BspMI | >Sse8387I | | | | | | >start_of_CMV_promoter/enhancer | | | | | | | | | | 60 GAATTCGAGC TTGCATGCCT GCAGGTCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC 120 TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG 180 CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG >NdeI | | 240 GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA 300 TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC >SnaBI >StyI | | >BsaA1 >NcoI | | | | 360 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG 420 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG 480 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA >SacI | >TATA_box_start_of_CMV_promoter | | | | 540 TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA >BsmB1 >BbsI | | >presumed_start_site_of_CMV_promoter | | | | | | 600 GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGACAC >BspE1 >XbaI >XhoI | | | >end_of_CMV_promoter_XbaI_site >start_of_SV40_splice_region | | | | | | | | | | 660 CGGGACCGAT CCAGCCTCCG GACTCTAGAG GATCCGGTAC TCGAGGAACT GAAAAACCAG >Psp5II | >Psp5II | >HpaI >EcoO109I | | >start_of_16S_RNA_intron >SV40_deletion_site_BamHI_site | | | | | | | | 720 AAAGTTAACT GGTAAGTTTA GTCTTTTTGT CTTTTATTTC AGGTCCCGGA TCCGGTGGTG >BseRI >StuI | | | >last_base_of_16S_RNA_intron | || | || 780 GTGCAAATCA AAGAACTGCT CCTCAGTGGA TGTTGCCTTT ACTTCTAGGC CTGTACGGAA >EagI | >NotI | >SacII | >end_of_SV40_sequence >NotI_cloning_site | | | | 840 GTGTTACTTC TGCTCTAAAA GCTGCGGAAT TGTACCCGCG GCCGCCAGCC ATGTATCCTT M Y P> >BciVI >SfiI >EcoNI | | | | | |900 ACGATGTACC AGACTATGCT GGGCCTCACG GGCCGATGTA TAGCCCGTAC TGCCTCACCC Y D V P D Y A G P> H G P> M Y S P Y C L T> >SexAI | 960 AGGATGAGTT CCACCCGTTT ATCGAGGCGC TGCTGCCTCA CGTCCGAGCC TTCTCCTACA Q D E F H P F I E A L L P H V R A F S Y> >BspMI | >PstI| | | >Sse8387I >ScaI | | | | | | 1020 CCTGGTTCAA CCTGCAGGCG CGGAAGCGCA AGTACTTCAA GAAGCACGAG AAGCGGATGT T W F N L Q A R K R K Y F K K H E K R M> >BssHII >BseRI | | | | 1080 CAAAGGACGA GGAGCGCGCA GTGAAGGACG AGCTGCTGGG CGAGAAGCCT GAGATCAAGC S K D E E R A V K D E L L G E K P E I K> >MscI >FspI | | | | 1140 AGAAGTGGGC ATCCCGGCTG TTGGCCAAGC TGCGCAAAGA CATCCGGCCC GAGTTCCGCG Q K W A S R L L A K L R K D I R P E F R> 1200 AGGACTTTGT GCTAACCATC ACGGGCAAGA AGCCCCCCTG CTGCGTGCTT TCCAACCCCG E D F V L T I T G K K P P C C V L S N P> >EcoNI | | 1260 ACCAGAAGGG CAAGATCCGG CGGATTGACT GCCTGCGCCA GGCTGACAAG GTGTGGCGGC D Q K G K I R R I D C L R Q A D K V W R> >Tth111I | >SexAI >ScaI | | | | | | 1320 TGGACCTGGT CATGGTGATT TTGTTTAAAG GGATCCCTTT GGAAAGTACT GATGGGGAGC L D L V M V I L F K G I P L E S T D G E> 1380 GGCTCTACAA GTCGCCCCAG TGCTCGAACC CCGGCCTGTG TGTCCAGCCA CATCACATTG R L Y K S P Q C S N P G L C V Q P H H I> >TfiI | >BspE1 >BspE1 | | | | | 1440 GAGTCACAAT CAAAGAACTG GACCTTTATC TGGCTTACTT TGTCCACACT CCGGAATCCG G V T I K E L D L Y L A Y F V H T P E S> 1500 GACAATCAGA TAGTTCAAAC CAGCAAGGAG ATGCGGACAT CAAACCACTG CCCAACGGGC G Q S D S S N Q Q G D A D I K P L P N G> >AflII | >end_of_NFI-X210_at_L | | | | 1560 ACTTAAGTTT CCAGGACTGC TTTGTGACGT CTGGGGTCTG GAATGTGACA GAGCTGGTGA H L S F Q D C F V T S G V W N V T E L V> >ApaI | >EcoO109I >XcmI | | | | | | 1620 GAGTATCACA GACTCCAGTT GCGACTGCAT CAGGGCCCAA CTTCTCACTG GCGGACCTGG R V S Q T P V A T A S G P N F S L A D L> 1680 AGAGCCCCAG CTACTACAAC ATAAATCAAG TGACCCTGGG AAGGCGGTCC ATCACCTCCC E S P S Y Y N I N Q V T L G R R S I T S> 1740 CTCCTTCCAC CAGCAGCACC AAGCGCCCCA AGTCCATCGA CGACAGTGAG ATGGAGAGTC P P S T S S T K R P K S I D D S E M E S> >EcoNI >EagI | | | | 1800 CAGTAGATGA TGTGTTCTAT CCTGGGACAG GCCGCTCTCC GGCCGCTGGC AGCAGCCAGT P V D D V F Y P G T G R S P A A G S S Q> >SmaI | >BstXI >XmaI| >BsgI >Eco47III | | | | | >X1,_X3_insert_123nt_maintaining_reading_frame | | | | | | | | | | | | | 1860 CTAGCGGATG GCCCAATGAC GTGGATGCAG GGAGCCCCCG GGCCACGGCA TCAGCGCTGC S S G W P N D V D A G S P R A T A S A L> 1920 ACTTCCCTTC CACGTCCATC ATCCAGCAGT CGAGCCCGTA TTTCACACAC CCAACCATCC H F P S T S I I Q Q S S P Y F T H P T I> >BstXI | >NcoI || >StyI || >XcmI| >Eco57I | || | | || | 1980 GCTACCACCA CCACCATGGG CAGGACTCGC TGAAGGAGTT TGTGCAGTTT GTGTGCTCTG R Y H H H H G Q D S L K E F V Q F V C S> >BsgI >BsmI >BseRI >NarI >AgeI | | | | | | >X3_has_148nt_insertion | | | || | | | | || | | 2040 ACGGCTCGGG TCAGGCCACC GGACAGCATT CACAACGACA GGCGCCTCCT CTGCCAACCG D G S G Q A T G Q H S Q R Q A P P L P T> >SmaI | >XmaI | | | | 2100 GTTTGTCAGC ATCGGATCCC GGGACGGCAA CTTTCTGAAC ATCCCACAGG AGTCTCAGTC G L S A S D P G T A T F *> >NheI | >BglII >NsiI| >SacII | | | | | | | | >BamHI | | | | | | | | >start_of_SV40_polyA_region | | | | | | | | | | | | 2160 CTAGATCTAT GCATAGCTAG CTAGGGCCGC GGGGATCCAG ACATGATAAG ATACATTGAT >BsmI | | 2220 GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT >HpaI >MunI | | | 2280 GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT >BsmI >XbaI | | | >end_of_SV40_polyA_region | | | | | 2340 TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTC GGATCCTCTA >BspMI | >AccI >PstI | >SphI | | | | >SalI >Sse8387I |>HindIII || | | | | || | | | | 2400 GAGTCGACCT GCAGGCATGC AAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA 2460 AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC >VspI | | 2520 TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC >TfiI | >PvuII >VspI | | | | | | | 2580 CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC >SapI | >EarI | | 2640 GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT >TfiI | | 2700 CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA >AflIII | >BspLU11I | >AflIII | | 2760 GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA 2820 AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT >DrdI | | 2880 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC >BciVI | | 2940 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC 3000 GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT >ApaLI | | 3060 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC 3120 CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG >AlwNI | | 3180 CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA 3240 GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC >Eco57I | | 3300 GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA 3360 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA 3420 GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC >RcaI | | 3480 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA 3540 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT 3600 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA >EamI105I | | 3660 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC >BsaI | >BsrDI | | 3720 AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC 3780 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG >Psp1406I | >VspI >FspI | | | | | | 3840 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC >BsrDI | | 3900 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC 3960 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG >PvuI | | 4020 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC 4080 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT >ScaI >BcgI | | | | 4140 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC >Bcg1 | | 4200 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC >XmnI | >Psp1406I | | 4260 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC >Eco57I | >ApaLI | | 4320 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC 4380 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA >EarI >SspI | | | | 4440 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT >BciVI | >RcaI | | | | 4500 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT >RcaI | | 4560 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA >EcoO109I >BsmB1 | | | | 4620 TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC >BsmB1 | | 4680 GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT >DrdI | | 4740 GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG >BstAPI | >ApaLI >NdeI | || | || 4800 CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA >NarI >FspI | | | 4860 CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC >PvuI >EarI >PvuII | | | | | | 4920 AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG 4980 GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGT Sequence Range: 1 to 4995 Enzyme #Cuts Positions AccI 1 2344 AflII 1 1502 AflIII 2 2720 2720 AgeI 1 2037 AlwNI 1 3136 ApaI 1 1597 ApaLI 3 3034 4280 4777 BbsI 1 600 Bcg1 1 4152 BcgI 1 4118 BciVI 3 844 2923 4450 BglII 1 2103 BsaA1 1 306 BsaI 1 3674 BseRI 3 730 1044 2017 BsgI 2 1842 1983 BsmB1 3 555 4603 4645 BsmI 3 2006 2189 2282 BspE1 3 617 1430 1437 BspLU11I 1 2720 BspMI 3 12 979 2356 BsrDI 2 3674 3848 BssHII 1 1034 BstAPI 1 4785 BstXI 2 1820 1935 DrdI 2 2828 4697 EagI 2 819 1780 EamI105I 1 3613 EarI 3 2604 4408 4896 Eco47III 1 1855 Eco57I 3 1971 3268 4280 EcoNI 3 897 1236 1765 EcoO109I 3 702 1593 4589 EcoRI 1 1 FspI 3 1113 3835 4858 HindIII 1 2361 HpaI 2 666 2268 MscI 1 1104 MunI 1 2277 NarI 2 2022 4836 NcoI 2 326 1934 NdeI 2 200 4784 NheI 1 2116 NotI 1 819 NsiI 1 2113 Psp1406I 2 3839 4212 Psp5II 2 702 702 PstI 3 23 976 2353 PvuI 2 3983 4879 PvuII 2 2544 4908 RcaI 3 3440 4448 4553 SacI 1 534 SacII 2 819 2130 SalI 1 2343 SapI 1 2604 ScaI 3 993 1307 4093 SexAI 2 960 1264 SfiI 1 869 SmaI 2 1839 2060 SnaBI 1 306 SphI 2 17 2359 Sse8387I 3 23 976 2353 SspI 1 4417 StuI 1 769 StyI 2 326 1934 TfiI 3 1434 2555 2695 Tth111I 1 1266 VspI 3 2491 2550 3785 XbaI 2 624 2337 XcmI 2 1604 1932 XhoI 1 640 XmaI 2 1837 2058 XmnI 1 4212