Tc1 End Sequences from Korswagen, Smits, Durbin, and
Plasterk
This page has a list of Tc1 alleles, resulting from a shotgun
sequencing approach. A description of the approach can be be found here, a
full manuscript with more details is being written. For more
information you can contact Rik
Korswagen (rkors@nki.nl) or Ronald Plasterk (rplas@ron.nki.nl).
To search the index...
All data will also become available as integrated part of acedb, with
the next release. All these alleles are defined (only) by the
sequences of the insertion sites (actually of one of the two
flanks). Therefore they will eventually all be mapped (semi-)
automatically by comparison to the C. elegans genome sequence, but at
the present time only the ones that map within the approximately 10%
of the genome that is sequenced are mapped. The mapped alleles are
certainly best viewed through acedb, for all the others all
information is contained within this page.
Note that these alleles are all derived from a PCR, and we can not
exclude that some of the insertions occurred late during the culturing
of the worm line in our lab, or even that they are somatic
insertions. Therefore there is no 100% guarantee that all of the
alleles can be found in the strain that is mentioned. We checked 10
alleles, and found all 10 to be real, so we would estimate these false
alleles to be less than 10%, but one should check each allele (e.g. by
a PCR, using a primer specific for this sequence) before using it for
further experimentation.
If you want to work with these alleles: the strains that contains them
are available from the CGC. We still have some DNA left that we used
to sequence the alleles, single stranded M13 DNA. If you need it, let
us know, but you could of course also amplify the sequence from
genomic worm DNA yourself.
Back to the genome page
Leon Avery
(Leon@eatworms.swmed.edu)
Last modified: Sat Jul 18 16:37:56 1998